SEC2-induced superantigen and antitumor activity is regulated through calcineurin
作者： Yanli Liu , Mingkai Xu , Huiwen Zhang , Xu Li , Zhencheng Su
DOI: 10.1007/S00253-013-4764-6
关键词: Pharmacology 、 Biology 、 Biological activity 、 Cell biology 、 NFAT 、 Superantigen 、 Cytotoxicity 、 Intracellular signal transduction 、 Immune system 、 Calcineurin 、 Signal transduction
摘要: Once the TCR-SAg-MHC II ternary complex is established, it triggers a variety of intracellular signal transduction pathways, which provoke extreme responses in immune system. However, signaling events that involved SAg-induced activation are not well understood. In this study, we demonstrated Ca2+/calcineurin (CaN)/nuclear factor activated T cells (NFAT) pathway was SEC2-induced activation, and selective blockade CaN by its inhibitor cyclosporine A (CsA) can completely inhibited T-cell stimulating potency. addition, selected an engineered SEC2 mutant named SAM-1 based on series biological activity tests, our further studies only confirmed gene transcription key substrates were proportional to SEC2/SAM-1-induced potency, but also suggested intensified Ca2+/CaN/NFAT induced resulted enhanced production cytokines cytotoxicity, finally elicit improved antitumor vivo.
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