CCAAT/Enhancer-Binding Protein β and δ Binding to CIITA Promoters Is Associated with the Inhibition of CIITA Expression in Response to Mycobacterium tuberculosis 19-kDa Lipoprotein

作者: Meghan E. Pennini , Yi Liu , Jianqi Yang , Colleen M. Croniger , W. Henry Boom

DOI: 10.4049/JIMMUNOL.179.10.6910

关键词: Nuclear proteinBiologyBinding siteRegulation of gene expressionPromoterGene isoformCcaat-enhancer-binding proteinsMolecular biologyChromatin immunoprecipitationCIITA

摘要: TLR2 signaling by Mycobacterium tuberculosis 19-kDa lipoprotein (LpqH) inhibits IFN-γ-induced expression of CIITA macrophages. Microarray analysis, quantitative RT-PCR, and Western blots showed that LpqH induced C/EBPβ C/EBPδ in kinetic correlation with inhibition expression. Of the isoforms, liver inhibitory protein (LIP) was notably liver-activating increased LpqH. Putative C/EBP binding sites were identified promoters I IV (pI pIV). (LIP protein) to biotinylated oligodeoxynucleotide containing pI or pIV sites, chromatin immunoprecipitation endogenous pIV. Constitutive LIP inhibited transfected cells. In summary, C/EBPδ, their pIV, macrophages, suggesting a role for as novel regulator

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