Femtomolar detection of single mismatches by discriminant analysis of DNA hybridization events using gold nanoparticles
作者： Xingyi Ma , Sang Jun Sim
DOI: 10.1039/C2AN36332A
关键词: DNA 、 DNA–DNA hybridization 、 Colloidal gold 、 Analyte 、 Helix 、 Nanosensor 、 Nanotechnology 、 Chemistry
摘要: Even though DNA-based nanosensors have been demonstrated for quantitative detection of analytes and diseases, hybridization events never numerically investigated further understanding DNA mediated interactions. Here, we developed a nanoscale platform with well-designed capture gold nanoprobes to precisely evaluate the events. The were mono-laid on glass probes fabricated via novel competitive conjugation method. two kinds combined in suitable orientation following target. We found that efficiency was markedly dependent electrostatic interactions between strands, which can be tailored by adjusting salt concentration incubation solution. Due much lower stability double helix formed mismatches, efficiencies single mismatched (MMT) perfectly matched (PMT) different. Therefore, obtained an optimized allowed discrimination MMT from PMT without stringent control temperature or pH. results indicated this ultrasensitive precise nanosensor diagnosis genetic diseases.
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