Control of adhesion, focal adhesion assembly, and differentiation of myoblasts by enzymatically crosslinked cell-interactive hydrogels
作者： Indong Jun , Kyung Min Park , Dong Yun Lee , Ki Dong Park , Heungsoo Shin
DOI: 10.1007/S13233-011-0909-6
关键词: Matrix (biology) 、 Biochemistry 、 Horseradish peroxidase 、 Tissue engineering 、 Adhesion 、 Focal adhesion assembly 、 Focal adhesion 、 Materials science 、 Self-healing hydrogels 、 Cell biology 、 Cell 、 Organic chemistry 、 Materials Chemistry 、 General Chemical Engineering 、 Polymers and Plastics
摘要: The development of an artificial matrix is critical as both a substrate to control the cell behavior and tool for examining roles cellular microenvironment in biology. This study developed cell-interactive hydrogels containing Arg-Gly-Asp (RGD) peptide, cross-linked via bio-inspired enzymatic processes using H2O2 horseradish peroxidase (HRP) initiators examined how they controlled myoblast functions. modulated adhesion proliferation myoblast, depending on peptide density. Furthermore, expression focal proteins myogenic differentiation were up-regulated significantly myoblasts cultured peptide-incorporated hydrogels. Therefore, novel hydrogel system can be used regulate function many tissue engineering applications.
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