G-protein activators induce a potassium conductance in murine macrophages.
作者： LeslieC. McKinney , ElaineK. Gallin
DOI: 10.1007/BF00240483
关键词: Apamin 、 Pertussis toxin 、 Extracellular 、 Biophysics 、 Cholera toxin 、 Charybdotoxin 、 Biochemistry 、 Chemistry 、 Hyperpolarization (biology) 、 Patch clamp 、 Intracellular
摘要: The whole-cell patch clamp technique was used to test whether intracellular application of G-protein activators affect ionic currents in murine macrophages. Both the J774.1 macrophage-like cell line and primary bone marrow derived macrophages were used. Cells bathed Na Hanks' solution intracellularly dialyzed (via pipette) with K Hanks (145mm KCl, 1mm), kinetics (no time-dependent inactivation). Intracellular GTP (500 μm), GDP cAMP (100 μm+0.5mm ATP), or IP3 (20 μm) did not induce current; 100 μm ATPγS activated a half-maximal amount current. Induction outward current by 10 GTPγS could be prevented pre-exposing cells pertussis toxin but cholera toxin. This is selective since (i) its induction accompanied hyperpolarization toward EK, even after Kir had “washed out”, (ii) it present >90% both extracellular Cl replaced isethionate, (iii) induced conductance absent when Ki completely Cs, reduced approximately 1/3 [K]i 1/3. Quinidine (1mm) 4-aminopyridine (10mm) inhibited current, apamin (1 charybdotoxin not.
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sci-hub.se 参考文章(41)
GM Feldman, S Ruhl, M Bickel, DS Finbloom, DH Pluznik, Regulation of interleukin-4 receptors on murine myeloid progenitor cells by interleukin-6. Blood. ,vol. 78, pp. 1678- 1684 ,(1991) , 10.1182/BLOOD.V78.7.1678.1678
Elaine K. Gallin, Leslie C. Mckinney, Chapter 6 Monovalent Ion Transport and Membrane Potential Changes during Activation in Phagocytic Leukocytes Current topics in membranes and transport. ,vol. 35, pp. 127- 152 ,(1990) , 10.1016/S0070-2161(08)60078-7
Joel Moss, Martha Vaughan, Adp-Ribosylation of Guanyl Nucleotide-Binding Regulatory Proteins by Bacterial Toxins Advances in Enzymology - and Related Areas of Molecular Biology. ,vol. 61, pp. 303- 379 ,(1988) , 10.1002/9780470123072.CH6
K G Lazzari, P Proto, E R Simons, Neutrophil hyperpolarization in response to a chemotactic peptide Journal of Biological Chemistry. ,vol. 265, pp. 10959- 10967 ,(1990) , 10.1016/S0021-9258(19)38542-4
M M Porasik-Lowes, G M Omann, Graded G-protein uncoupling by pertussis toxin treatment of human polymorphonuclear leukocytes. Journal of Immunology. ,vol. 146, pp. 1303- 1308 ,(1991)
P G Polakis, R J Uhing, R Snyderman, The formylpeptide chemoattractant receptor copurifies with a GTP-binding protein containing a distinct 40-kDa pertussis toxin substrate. Journal of Biological Chemistry. ,vol. 263, pp. 4969- 4976 ,(1988) , 10.1016/S0021-9258(18)68882-9
J.T. Wu, Q.H. Gong, R.H. Chou, S.J. Wieland, Ca(2+)-insensitive modulation of a K+ conductance by inositol polyphosphates Journal of Biological Chemistry. ,vol. 266, pp. 14893- 14895 ,(1991) , 10.1016/S0021-9258(18)98561-3
E R Stanley, P M Heard, Factors regulating macrophage production and growth. Purification and some properties of the colony stimulating factor from medium conditioned by mouse L cells. Journal of Biological Chemistry. ,vol. 252, pp. 4305- 4312 ,(1977) , 10.1016/S0021-9258(17)40265-1
T Higashijima, M P Graziano, H Suga, M Kainosho, A G Gilman, 19F and 31P NMR spectroscopy of G protein alpha subunits. Mechanism of activation by Al3+ and F-. Journal of Biological Chemistry. ,vol. 266, pp. 3396- 3401 ,(1991) , 10.1016/S0021-9258(19)67806-3
T E DeCoursey, E R Jacobs, Mechanisms of potassium channel block in rat alveolar epithelial cells. Journal of Pharmacology and Experimental Therapeutics. ,vol. 255, pp. 459- 472 ,(1990)