A Green-Light-Responsive System for the Control of Transgene Expression in Mammalian and Plant Cells
作者： Claire Chatelle , Rocio Ochoa-Fernandez , Raphael Engesser , Nils Schneider , Hannes M. Beyer
DOI: 10.1021/ACSSYNBIO.7B00450
关键词: Endogeny 、 Gene expression 、 Bacterial transcription 、 Thermus thermophilus 、 Cell biology 、 Transgene 、 Plant cell 、 Synthetic biology 、 Biology 、 Optogenetics
摘要: The ever-increasing complexity of synthetic gene networks and applications biology requires precise orthogonal expression systems. Of particular interest are systems responsive to light as they enable the control dynamics with unprecedented resolution in space time. While broadly used mammalian backgrounds, however, optogenetic approaches plant cells still limited due interference activating endogenous photoreceptors. Here, we describe development first light-responsive system for targeted that responds green range spectrum which photoreceptors have minimal activity. We engineered a based on light-sensitive bacterial transcription factor CarH its cognate DNA operator sequence CarO from Thermus thermophilus cells. was functional various cel...
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