Methods and compositions for in vitro amplification of extrachromosomal nucleic acid
作者： Lars-Erik Peters , Elaine Best , Allan Roberts , David Latorra , Preston Hradecky
DOI:
关键词: Extrachromosomal DNA 、 Biology 、 Biochemistry 、 In vitro 、 Molecule 、 Low ionic strength 、 Polymerization 、 Nucleic acid 、 Ligation
摘要: Compositions and methods are provided for the in vitro amplification of nucleic acid and, particular, extrachromosomal molecules having a molecular weight one kilobase or greater. The design use primers, low ionic strength buffer, co-solvent containing combined polymerization/ligation reaction buffers described this regard.
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