Hypoxic Induction of Anoxia Tolerance in Root Tips of Zea mays
作者： James Johnson , B. Greg Cobb , Malcolm C. Drew
DOI: 10.1104/PP.91.3.837
关键词: Oxygene 、 Biochemistry 、 Adenine nucleotide 、 Anaerobic exercise 、 Adenylate kinase 、 Alcohol dehydrogenase 、 Oxygen 、 Biology 、 Fermentation 、 Animal science 、 Energy charge
摘要: When root tips of fully aerobic, intact maize (Zea mays L.) seedlings are made anaerobic, viability normally is only 24 hours or less at 25°C. We find that can be extended to least 96 if given a hypoxic pretreatment for 18 by sparging the solution with 4% O2 in nitrogen (v/v) before anoxia. Fully aerobic (sparged 40% O2) had very low alcohol dehydrogenase (ADH) activity (per gram fresh weight), and level remained under In hypoxically pretreated roots, however, high levels ADH were induced, rose further during initial anoxia, then about 20 times controls O2. roots sparged air (21% was three Improved associated maintenance energy metabolism (ATP concentration, total adenylates, adenylate charge). Roots not lost 94% adenylates ATP The relation between induced activity, metabolism, improved anoxia-tolerance acclimated discussed.
plantphysiol.org参考文章(21)
W. Armstrong, Aeration in Higher Plants Advances in Botanical Research. ,vol. 7, pp. 225- 332 ,(1980) , 10.1016/S0065-2296(08)60089-0
D. J. Greenwood, Soil Aeration and Plant Growth Advances in Agronomy. ,vol. 18, pp. 57- 106 ,(1966) , 10.1016/S0065-2113(08)60648-3
P M Kelley, M Freeling, Anaerobic expression of maize glucose phosphate isomerase I. Journal of Biological Chemistry. ,vol. 259, pp. 673- 677 ,(1984) , 10.1016/S0021-9258(17)43714-8
Pierre H. Saglio, Alain Pradet, Soluble Sugars, Respiration, and Energy Charge during Aging of Excised Maize Root Tips. Plant Physiology. ,vol. 66, pp. 516- 519 ,(1980) , 10.1104/PP.66.3.516
Justin K. M. Roberts, Fernando H. Andrade, Irvin C. Anderson, Further Evidence that Cytoplasmic Acidosis Is a Determinant of Flooding Intolerance in Plants Plant Physiology. ,vol. 77, pp. 492- 494 ,(1985) , 10.1104/PP.77.2.492
Mary E. Rumpho, Alain Pradet, Abdul Khalik, Robert A. Kennedy, Energy charge and emergence of the coleoptile and radicle at varying oxygen levels in Echinochloa crus-galli Physiologia Plantarum. ,vol. 62, pp. 133- 138 ,(1984) , 10.1111/J.1399-3054.1984.TB00360.X
Martin M. Sachs, Michael Freeling, Ronald Okimoto, The anaerobic proteins of maize. Cell. ,vol. 20, pp. 761- 767 ,(1980) , 10.1016/0092-8674(80)90322-0
Lorna S. Monk, Roland Brändle, Adaptation of Respiration and Fermentation to Changing Levels of Oxygen in Rhizomes of Schoenoplectus lacustris (L.) Palla and Its Significance to Flooding Tolerance Zeitschrift für Pflanzenphysiologie. ,vol. 105, pp. 369- 374 ,(1982) , 10.1016/S0044-328X(82)80033-0
J. K. Roberts, J. Callis, O. Jardetzky, V. Walbot, M. Freeling, Cytoplasmic acidosis as a determinant of flooding intolerance in plants Proceedings of the National Academy of Sciences of the United States of America. ,vol. 81, pp. 6029- 6033 ,(1984) , 10.1073/PNAS.81.19.6029
Loveday E.T. Jenkin, Tom Ap Rees, Effects of anoxia and flooding on alcohol dehydrogenase in roots of Glyceria maxima and Pisum sativum Phytochemistry. ,vol. 22, pp. 2389- 2393 ,(1983) , 10.1016/0031-9422(83)80125-3