sn-glycerol-3-phosphate acyltransferase tubule formation is dependent upon heat shock proteins (htpR).

摘要: Abstract Overexpression of the Escherichia coli sn-glycerol-3-phosphate (glycerol-P) acyltransferase, an integral membrane protein, causes formation ordered arrays enzyme in vitro. The these tubular structures did not occur E. strain bearing a mutation htpR gene, regulatory gene for heat shock response. htpR165 was shown by genetic analysis to be lesion responsible blockage tubule formation. Similar amounts glycerol-P acyltransferase were produced isogenic htpR+ and strains, ruling out effect on expression acyltransferase. Further, phospholipid metabolism altered either after induction synthesis. Increased synthesis caused partial response which dependent upon wild type gene. proteins induced identified as groEL dnaK products two-dimensional gels. These two have been implicated assembly bacteriophage coats. appear essential