Genomic responses to hepatitis B virus (HBV) infection in primary human hepatocytes

摘要: // Pierre-Benoit Ancey 1 , Barbara Testoni 2 Marion Gruffaz Marie-Pierre Cros Geoffroy Durand 3 Florence Le Calvez-Kelm David Durantel Zdenko Herceg Hector Hernandez-Vargas Epigenetics Group, International Agency for Research on Cancer (IARC), Lyon, France INSERM U1052, Molecular Physiopathology and New Treatments of Viral Hepatitis, Centre de Recherche en Cancerologie (CRCL), Genetic Susceptibility Correspondence to: Hernandez-Vargas, e-mail: vargash@iarc.fr Keywords: HBV, hepatitis, methylome, HM450, transcriptome Received: August 17, 2015      Accepted: October 14, Published: November 02, 2015 ABSTRACT infections are able to modify the host’s cellular programs, with DNA methylation being a biological intermediate in this process. The extent which viral deregulate gene expression is not fully understood. In case Hepatitis B virus (HBV), there evidence an interaction between proteins host machinery. We studied ability HBV using naturally infected primary human hepatocytes better mimic clinical setting. Gene was especially sensitive culture conditions, independently infection. However, we identified non-random changes occurring specifically upon There little correlation changes, more marker time-dependent induced by HBV. contrast, set differentially methylated sites appeared early were stable across time course experiment. Finally, HBV-induced defined specific chromatin context characterized CpG-poor regions outside promoters. These data support modulate cell programs. addition, it may serve as reference studies addressing genome-wide consequences infection hepatocytes.