STED Nanoscopy in Living Cells Using Fluorogen Activating Proteins
作者： James A. J. Fitzpatrick , Qi Yan , Jochen J. Sieber , Marcus Dyba , Ulf Schwarz
DOI: 10.1021/BC900249E
关键词: Cell biology 、 Nanotechnology 、 STED microscopy 、 Intracellular 、 Chemistry 、 Cell 、 Extracellular
摘要: We demonstrate the effectiveness of a genetically encoded Malachite Green (MG) binding fluorogen activating protein (FAP) for live cell stimulated emission depletion nanoscopy (STED). Both extracellular and intracellular FAPs were tested in living cells using fluorogens with either membrane expressed FAP or as an FAP-actin fusion. Structures FWHM 110-122nm observed. Depletion data however suggests resolution 70nm given instrument.
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