DNA mediated assembly of quantum dot-protoporphyrin IX FRET probes and the effect of FRET efficiency on ROS generation.
作者： Seema Singh , Anirban Chakraborty , Vandana Singh , Aniruddha Molla , Sahid Hussain
DOI: 10.1039/C4CP05306K
关键词: Quantum dot 、 Förster resonance energy transfer 、 Absorption spectroscopy 、 Photodynamic therapy 、 Conjugated system 、 Chemistry 、 Photochemistry 、 Acceptor 、 Protoporphyrin IX 、 Photosensitizer
摘要: Photodynamic therapy (PDT) involves generation of reactive oxygen species (ROS) by the irradiation a photosensitizer. Controlled and targeted release ROS photosensitizer is crucial in PDT. For achieving controlled ROS, ZnSe/ZnS quantum dot (QD) donor protoporphyrin IX (Pp IX) acceptor based fluorescence resonance energy transfer (FRET) probe reported here. The QDs Pp are assembled either direct conjugation or through DNA hybridization. Complementary strands individually conjugated to amide coupling. Due overlap emission spectrum absorption IX, efficient from was observed both cases. FRET efficiency quantitatively evaluated steady-state time-resolved spectroscopy compared between QD–Pp conjugate QD–DNA–Pp assembly at various ratios. Since single QD can harbor multiple number IX–DNA counterparts hybridization, found increase with acceptors. studied for pairs be affected time donor.
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