METHOD AND SYSTEM FOR SYNTHESIZING OLIGONUCLEOTIDE
作者： Huang Xiaoluo , Feng Lihe , Zhang Wenwei , Zhou Chao , Xu Xun
DOI:
关键词: Monomer 、 Combinatorial chemistry 、 Nucleotide 、 Chemistry 、 Phosphoramidite 、 Nucleic acid 、 Reagent 、 Oligonucleotide
摘要: Provided is a method and system for synthesizing nucleic acid with ''a synthesis pool''. The based on phosphoramidite solid-phase of in four-step cycle, whereindeprotection, coupling, capping oxidation are performed first reaction vessel, second third fourth vessel that independent one another. relates to using multi-base nucleotide as monomer, such having longer chain can be rapidly synthesized, the error rate low, reagent repeatedly used so efficiency greatly improved.
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