Naturally acquired bovine besnoitiosis: histological and immunohistochemical findings in acute, subacute, and chronic disease.

作者: M. C. Langenmayer , N. S. Gollnick , M. Majzoub-Altweck , J. C. Scharr , G. Schares

DOI: 10.1177/0300985814541705

关键词: HistologyH&E stainSubclinical infectionAnatomyPathogenesisBesnoitia besnoitiPathologyBiologyCystBesnoitiaBesnoitiosis

摘要: The pathogenesis of bovine besnoitiosis, a disease increasing concern within Europe, is still incompletely understood. In this study, progression after natural infection with the causative apicomplexan Besnoitia besnoiti was monitored in histological skin sections 5 individual female cattle over time. High-frequency sampling 2 mild and severe acute, subacute, chronic as well from 1 animal during subclinical disease, enabled documentation beginning disease. Skin were stained hematoxylin eosin Giemsa, periodic acid–Schiff reaction, anti-Besnoitia immunohistochemistry. all 4 clinically affected animals, tachyzoite-like endozoites could be detected for first time by immunohistochemistry, tissue cyst evolution monitored. Besnoitiosis-associated lesions not showing course. Because inconsistency nomenclature layers literature, new B. wall proposed: cysts consist hypertrophied host cell enlarged nuclei, an intracytoplasmic parasitophorous vacuole bradyzoites, sometimes vacuolated inner wall, outer more developed cysts. Inner walls can readily distinguished using special stains. animal, extracystic zoites immunohistochemically stage. At necropsy, severely cows displayed large numbers variety tissues, including corium claws, contributing mainly to development laminitis these cases.

参考文章(48)
M. Majzoub, N.S. Gollnick, W. Hermanns, A. Rostaher, Gereon Schares, W. Breuer, Ein Ausbruch von Besnoitiose bei Rindern in Deutschland: pathomorphologische, ultrastrukturelle und molekularbiologische Untersuchungen Veterinary medicine Austria : WTM. ,vol. 97, pp. 9- 15 ,(2010)
M. Lesser, U. Braun, P. Deplazes, B. Gottstein, M. Hilbe, W. Basso, First cases of besnoitiosis in cattle in Switzerland Schweizer Archiv Fur Tierheilkunde. ,vol. 154, pp. 469- 474 ,(2012) , 10.1024/0036-7281/A000389
T.A. Nobel, M. Neumann, U. Klopfer, S. Perl, Kystes de Besnoîtia besnoîti dans les organes génitaux de la vache Bulletin De L Academie Veterinaire De France. pp. 569- ,(1977) , 10.4267/2042/66051
Gianfranco Militerno, G. Schares, M. Majzoub, Patrizia Bassi, N. S. Gollnick, Arcangelo Gentile, Su di un episodio di Besnoitiosi bovina in Italia pp. 3- 16 ,(2010)
J. A. W. Coetzer, R. C. Tustin, Infectious diseases of livestock Oxford University Press. ,(2004)
K. C. A. Sehulz, A report on naturally acquired Besnoitiosis in bovines with special reference to its pathology Journal of the South African Veterinary Medical Association. ,vol. 31, pp. 21- 35 ,(1960)
Boris Hinz, Sem H. Phan, Victor J. Thannickal, Andrea Galli, Marie-Luce Bochaton-Piallat, Giulio Gabbiani, The Myofibroblast: One Function, Multiple Origins American Journal of Pathology. ,vol. 170, pp. 1807- 1816 ,(2007) , 10.2353/AJPATH.2007.070112
J. W. Pols, Studies on bovine besnoiti-osis with special reference to the aetiology. Onderstepoort Journal of Veterinary Research. ,vol. 28, pp. 265- 356 ,(1960)
R. D. Bigalke, Besnoitiosis and Globidiosis Springer, Dordrecht. pp. 429- 442 ,(1981) , 10.1007/978-94-011-9034-3_34
R.M. McCully, R.D. Bigalke, P.A. Basson, Observations on the pathogenesis of bovine and antelope strains of Besnoitia besnoiti (Marotel, 1912) infection in cattle and rabbits. Onderstepoort Journal of Veterinary Research. ,vol. 37, pp. 105- 126 ,(1970)