作者: M. C. Langenmayer , N. S. Gollnick , M. Majzoub-Altweck , J. C. Scharr , G. Schares
关键词: Histology 、 H&E stain 、 Subclinical infection 、 Anatomy 、 Pathogenesis 、 Besnoitia besnoiti 、 Pathology 、 Biology 、 Cyst 、 Besnoitia 、 Besnoitiosis
摘要: The pathogenesis of bovine besnoitiosis, a disease increasing concern within Europe, is still incompletely understood. In this study, progression after natural infection with the causative apicomplexan Besnoitia besnoiti was monitored in histological skin sections 5 individual female cattle over time. High-frequency sampling 2 mild and severe acute, subacute, chronic as well from 1 animal during subclinical disease, enabled documentation beginning disease. Skin were stained hematoxylin eosin Giemsa, periodic acid–Schiff reaction, anti-Besnoitia immunohistochemistry. all 4 clinically affected animals, tachyzoite-like endozoites could be detected for first time by immunohistochemistry, tissue cyst evolution monitored. Besnoitiosis-associated lesions not showing course. Because inconsistency nomenclature layers literature, new B. wall proposed: cysts consist hypertrophied host cell enlarged nuclei, an intracytoplasmic parasitophorous vacuole bradyzoites, sometimes vacuolated inner wall, outer more developed cysts. Inner walls can readily distinguished using special stains. animal, extracystic zoites immunohistochemically stage. At necropsy, severely cows displayed large numbers variety tissues, including corium claws, contributing mainly to development laminitis these cases.