Intrinsically stealth fluorescent organic nanoparticles made of articulated bis-dipolar dyes for single particle tracking

摘要: Luminescent nanoparticles are becoming fundamental tools to the field of bioimaging. The optimization their size, brightness and stability is key for applications ranging from contrast agent assisted surgery diagnosis therapeutics. A plethora formulations have been documented which can be split into inorganic, organic hybrid categories. While each class has own advantages limitations, controlling interactions occurring between cellular membranes utmost importance. In particular, a major challenge various applications, especially molecular imaging membrane receptors, prevent non-specific interactions. Towards this goal, popular strategies based on coating with PEG or zwitterionic moieties developed yield stealth nanoparticles. study, we present series spontaneously water-soluble These fluorescent nanoparticles, made original articulated bis-dipolar dyes, show vanishing living cells as bare Moreover, thanks stability, they tracked isolated single emitters in aqueous environments. thus hold promise specific such neuronal after bioconjugation dedicated targeting agents.