Increased expression of chemokine receptor CCR3 and its ligands in ulcerative colitis: the role of colonic epithelial cells in in vitro studies

摘要: Human colonic epithelial cells express T helper type 1 (Th1)-associated chemoattractants, yet little is known about the production of Th2-associated chemoattractants. CCL11/eotaxin-1, CCL24/eotaxin-2 and CCL26/eotaxin-3 are to attract CCR3-expressing, Th2-polarized lymphocytes. We studied constitutive inflammation-induced expression CCR3 together with its ligands in colon peripheral blood patients inflammatory bowel disease (IBD) by flow cytometry, reverse transcription–polymerase chain reaction (RT–PCR) enzyme-linked immunosorbent assay (ELISA). further defined regulated these chemokines RT–PCR ELISA using cultured human cell lines. A higher fraction lymphocytes were found be positive for ulcerative colitis (UC) compared Crohn’s (CD), while almost no CCR3(+) normal controls (NC). Similarly, more frequent was observed biopsies from UC, regardless activity, when CD or NCs. Serum CCL11/eotaxin-1 increased significantly UC (306 ± 87 pg/ml) less so (257 43 pg/ml), whereas CCL24/eotaxin-2, only UC. Colonic three minimal NCs but high diseases (especially UC) independent activity. Th2, a lesser extent Th1, cytokines able induce all eotaxins culture. over-expression would appear characteristic The suggests that epithelium can play role modulating pathological cell-mediated mucosal inflammation.