Assembly of the alpha-toxin-hexamer of Staphylococcus aureus in the liposome membrane.
作者： H. Ikigai , T. Nakae
DOI: 10.1016/S0021-9258(18)61631-X
关键词: Unsaturated fatty acid 、 Fluorescence 、 Chromatography 、 Phosphatidylethanolamine 、 Membrane lipids 、 Liposome 、 Random hexamer 、 Tryptophan 、 Phosphatidylcholine 、 Chemistry 、 Cell biology 、 Biochemistry 、 Molecular biology
摘要: It has been shown that the access of alpha-toxin Staphylococcus aureus to target membrane and assembly hexamer can be monitored independently by respectively measuring fluorescence energy transfer from tryptophan residue(s) toxin dansylated phosphatidylethanolamine in liposome increment at 336 nm (Ikigai, H., Nakae, T., (1987) J. Biol. Chem. 262, 2150-2155). Measurement these parameters under various conditions showed following results: when phosphatidylcholine (PC) liposomes composed saturated fatty acids were mixed with toxin, occurred below, at, above transition temperature lipid, but change was never detectable; PC-liposomes containing unsaturated used, both observed. These results suggested toxin-membrane interaction occurs PC-membranes and/or oligomerization only presence PC acid(s). This conclusion supported quantitative determination toxin-hexamer leakage carboxyfluorescein similar above.
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