How ribosomes select initiator regions in mRNA: base pair formation between the 3' terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli.

摘要: Abstract Initiation complexes formed by E. coli ribosomes in the presence of 32P-labeled A protein initiator region from R17 bacteriophage Rna have been treated with colicin E3 and disassembled exposure to 1% sodium dodecyl sulfate. Electrophoresis on 9% polyacrylamide gels reveals a dissociable complex containing 30-nucleotide-long messenger fragment 50-nucleotide-long fragment, which arises 3' terminus 16S RNA. The is pure RNA-RNA hybird; it apparently maintained seven-base complementarity between two RNA fragments. Detection this mRNA-rRNA strongly supports hypothesis that during initiation step biosynthesis end base pairs polypurine stretch common regions mRNAs. implications our findings respect molecular mechanism site selection mRNA binding ribosomes, role rRNA ribosome function, species specificity translation are explored.