Cyanine dye as monitor of membrane potentials in Escherichia coli cells and membrane vesicles.
作者： Lucienne LETELLIER , Emanuel SHECHTER
DOI: 10.1111/J.1432-1033.1979.TB04259.X
关键词: Membrane potential 、 Analytical chemistry 、 Energy source 、 Fluorescence 、 Cyanine 、 Vesicle 、 Chemistry 、 Biophysics 、 Iodide 、 Quenching (fluorescence) 、 Potassium
摘要: The fluorescence response of a positively charged cyanine dye: 3,3'-dimethylindodicarbocyanine iodide can be specifically related to the generation in Escherichia coli cells and E. membrane vesicles an electrical potential induced either by substrate oxidation or artificially imposed potassium diffusion gradient. energy-dependent quenching dye correlates well with known effect on delta phi of: various energy sources, external pH solute accumulation. Thus, vesicles, increases from succinate D-lactate, ascorbate/phenazine methosulfate parallels increasing ability these electron donors generate phi. In is only weakly dependent pH, whereas cells, pH. Lactose accumulation results partial utilization A calibration terms has been determined using valinomycin-induced potential.
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