NITRO-REDUCTASE FROM A NOCARDIA SP.

摘要: The activity ofNocardia V. nitro-reductase appears to vary considerably during the life cycle of culture. optimal conditions found for formation highest amount enzyme per unit dry weight cell were: incubation organism in a medium containing salts, glucose, and sodium glutamate at pH 7.4 28–30 hr about 29–30 C. TheNocardia is constitutive contrast adaptive nitrate- nitrite-reductases other organisms. requires sulphydryl compound uses reduced pyridine nucleotides as electron donor reduction nitro-groups. only product when usingp-dinitrobenzene substrate isp-nitroaniline. This has been identified by electrophoresis, spectroscopy, melting point, chemical derivatives elementary analysis. No evidence obtained which indicates nature intermediate steps biological