Titration of Activated Bovine Factor X
作者： Robert L. Smith
DOI: 10.1016/S0021-9258(19)44125-2
关键词: Chromatography 、 Titration 、 Factor X 、 Active site 、 Substrate (chemistry) 、 Zymogen 、 Viper Venoms 、 Biochemistry 、 Chemistry
摘要: Abstract p-Nitrophenyl-p'-guanidinobenzoate is a suitable substrate for the titration of purified bovine Factor X which has been activated by coagulant protein from Russell's viper venom. Reaction Xa with p-nitrophenyl-p'-guanidinobenzoate occurs at site responsible clotting activity Xa. Activation venom yields single active per 53,000 daltons zymogen.
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