Linc00152 promotes malignant progression of glioma stem cells by regulating miR-103a-3p/FEZF1/CDC25A pathway
作者： Mingjun Yu , Yixue Xue , Jian Zheng , Xiaobai Liu , Hai Yu
DOI: 10.1186/S12943-017-0677-9
关键词: PI3K/AKT/mTOR pathway 、 Stem cell 、 CDC25A 、 Biology 、 Protein kinase B 、 Glioma 、 Cancer research 、 Cell growth 、 microRNA 、 Gene knockdown
摘要: Glioma is one of the most frequent intracranial malignant tumors. LncRNAs have been identified as new modulators in origination and progression glioma. Quantitative real-time PCR were conducted to evaluate expression linc00152 miRNA-103a-3p glioma tissues cells. Western blot used determine FEZF1 CDC25A Stable knockdown or over-expression miR-103a-3p stem cells (GSCs) established explore function GSCs. Further, luciferase reports investigate correlation between miR-103a-3p. Cell Counting Kit-8, transwell assays, flow cytometry GSC biological behaviors. ChIP assays employed ascertain correlations CDC25A. Linc00152 was up-regulated well Knockdown inhibited cell proliferation, migration invasion, while promoted apoptosis. regulated behavior GSCs by binding miR-103a-3p, which functions a tumor suppressor. In addition, down-regulated forebrain embryonic zinc finger protein 1 (FEZF1), direct target played an oncogenic role elevated promoter activities gene division cycle 25A (CDC25A). activated PI3K/AKT pathways, Linc00152/miR-103a-3p/FEZF1/CDC25A axis plays novel regulating GSCs, may be potential therapeutic strategy for therapy.
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