作者: Nelson C. Soares , Rita Francisco , Jesus Maria Vielba , Cândido Pinto Ricardo , Phil A. Jackson
DOI: 10.1021/PR8009353
关键词: Respiratory burst 、 Cell biology 、 Jasmonic acid 、 Medicago truncatula 、 Apoplast 、 Biochemistry 、 Arabidopsis 、 Signal transduction 、 Proteome 、 Biology 、 Extracellular matrix
摘要: Early wound-related changes in the leaf apoplast proteome of Medicago truncatula have been characterized by 2-DE and MALDI-TOF/TOF differential expression 28/110 extracellular proteins could be reproducibly observed 6 h after wounding. Wounding induced an initial (0−30 min) burst O2−, followed a later (3−6 h) production O2− H2O2. The infiltration 5 μM DPI ≤ 3 min wounding inhibited both phases oxidative suppressed wound-regulated 9/28 proteins. infiltrated 15 only partially early was ineffective suppressing these This strongly suggests that wounded Medicago, rapid is required for mobilizing downstream h), several Further studies with exogenous sources ROS supported regulation within early, ROS-signaling events. study forms ...