Associating wound-related changes in the apoplast proteome of Medicago with early steps in the ROS signal-transduction pathway.

作者: Nelson C. Soares , Rita Francisco , Jesus Maria Vielba , Cândido Pinto Ricardo , Phil A. Jackson

DOI: 10.1021/PR8009353

关键词: Respiratory burstCell biologyJasmonic acidMedicago truncatulaApoplastBiochemistryArabidopsisSignal transductionProteomeBiologyExtracellular matrix

摘要: Early wound-related changes in the leaf apoplast proteome of Medicago truncatula have been characterized by 2-DE and MALDI-TOF/TOF differential expression 28/110 extracellular proteins could be reproducibly observed 6 h after wounding. Wounding induced an initial (0−30 min) burst O2−, followed a later (3−6 h) production O2− H2O2. The infiltration 5 μM DPI ≤ 3 min wounding inhibited both phases oxidative suppressed wound-regulated 9/28 proteins. infiltrated 15 only partially early was ineffective suppressing these This strongly suggests that wounded Medicago, rapid is required for mobilizing downstream h), several Further studies with exogenous sources ROS supported regulation within early, ROS-signaling events. study forms ...

参考文章(67)
M. Orozco-Cardenas, C. A. Ryan, Hydrogen peroxide is generated systemically in plant leaves by wounding and systemin via the octadecanoid pathway Proceedings of the National Academy of Sciences of the United States of America. ,vol. 96, pp. 6553- 6557 ,(1999) , 10.1073/PNAS.96.11.6553
Charlotte J. Song, Iris Steinebrunner, Xuanzhi Wang, Stephen C. Stout, Stanley J. Roux, Extracellular ATP Induces the Accumulation of Superoxide via NADPH Oxidases in Arabidopsis Plant Physiology. ,vol. 140, pp. 1222- 1232 ,(2006) , 10.1104/PP.105.073072
Andrej Shevchenko, Shamil Sunyaev, Alexander Loboda, Anna Shevchenko, Peer Bork, Werner Ens, Kenneth G. Standing, Charting the Proteomes of Organisms with Unsequenced Genomes by MALDI-Quadrupole Time-of-Flight Mass Spectrometry and BLAST Homology Searching Analytical Chemistry. ,vol. 73, pp. 1917- 1926 ,(2001) , 10.1021/AC0013709
Dasharath Prasad Lohar, Sajeet Haridas, J. Stephen Gantt, Kathryn A. VandenBosch, A transient decrease in reactive oxygen species in roots leads to root hair deformation in the legume-rhizobia symbiosis. New Phytologist. ,vol. 173, pp. 39- 49 ,(2007) , 10.1111/J.1469-8137.2006.01901.X
José A Hernández, Maria Angeles Ferrer, Ana Jiménez, Alfonso Ros Barceló, Francisca Sevilla, None, ANTIOXIDANT SYSTEMS AND O2.?/H2O2 PRODUCTION IN THE APOPLAST OF PEA LEAVES. ITS RELATION WITH SALT-INDUCED NECROTIC LESIONS IN MINOR VEINS Plant Physiology. ,vol. 127, pp. 817- 831 ,(2001) , 10.1104/PP.010188
Ron Mittler, Sandy Vanderauwera, Martin Gollery, Frank Van Breusegem, Reactive oxygen gene network of plants Trends in Plant Science. ,vol. 9, pp. 490- 498 ,(2004) , 10.1016/J.TPLANTS.2004.08.009
Gitta Frahry, Peter Schopfer, Inhibition of O2-reducing activity of horseradish peroxidase by diphenyleneiodonium Phytochemistry. ,vol. 48, pp. 223- 227 ,(1998) , 10.1016/S0031-9422(98)00004-1
Phil A.P. Jackson, Carla I.R. Galinha, Cristina S. Pereira, Ana Fortunato, Nelson C. Soares, Sara B.Q. Amâncio, Cândido P. Pinto Ricardo, Rapid deposition of extensin during the elicitation of grapevine callus cultures is specifically catalyzed by a 40-kilodalton peroxidase. Plant Physiology. ,vol. 127, pp. 1065- 1076 ,(2001) , 10.1104/PP.010192