Leucocyte locomotion and chemotaxis: The influence of divalent cations and cation ionophores☆

摘要: Abstract Human blood neutrophil leucocytes and monocytes incubated in the absence of Ca2+ Mg2+ showed reduced, but still substantial migration into micropore filters towards chemotactic agents, compared with cells migrating a divalent cation-rich medium. This reduction could be reversed by adding low doses cation ionophores (X537A or A23187) to Ca2+- Mg2+-free medium which suggests that media depleted extracellular cations can make use intracellular exchange necessary for locomotion is facilitated ionophores. At higher doses, inhibited locomotion, as did procaine reduces membrane permeability cations. Little effect K+ depletion ouabain on leucocyte was noted.