Apple Derived Cellulose Scaffolds for 3D Mammalian Cell Culture

摘要: There are numerous approaches for producing natural and synthetic 3D scaffolds that support the proliferation of mammalian cells. better represent cellular microenvironment have many potential applications in vitro vivo. Here, we demonstrate cellulose produced by decellularizing apple hypanthium tissue can be employed culture NIH3T3 fibroblasts, mouse C2C12 muscle myoblasts human HeLa epithelial We show these cells adhere, invade proliferate scaffolds. In addition, biochemical functionalization or chemical cross-linking to control surface biochemistry and/or mechanical properties scaffold. The retain high viability even after 12 continuous weeks achieve cell densities comparable with other scaffold materials. Apple derived easily produced, inexpensive originate from a renewable source. Taken together, results naturally offer complementary approach existing techniques environment.