Variation in antioxidant properties and metabolites during flower maturation of Flos Lonicerae Japonicae flowers

作者: Maorun Fu , Qingli Qu , Hongfei Dai

DOI: 10.1007/S00217-014-2378-6

关键词: DPPHChlorogenic acidRutinFlosFood scienceCaffeic acidSuperoxideStage iiBiochemistryChemistryAntioxidant

摘要: Antioxidant properties of methanol extracts from Flos Lonicerae Japonicae (FLJ) flowers during maturation were determined with different antioxidant assays, including activity in emulsion system, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, superoxide anion scavenging and reducing power. metabolites, such as chlorogenic acid, caffeic rutin, 3,5-O-dicaffeoylquinic acid (3,5-O-diCQA) 3,4-O-diCQA, also identified analyzed by DPPH–HPLC–DAD–TOF/MS method. Significant variation related compounds was observed between maturity stages, the strongest at stage I accompanying highest content compounds. Chlorogenic its derivatives main FLJ flowers, accounting for more than 90 % total bioactive molecules. In summary, bud-developing-stage (stage II) possess functional benefit.

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