Tissue Kallikrein in Rat Brain and Pituitary: Regional Distribution and Estrogen Induction in the Anterior Pituitary

摘要: We have detected tissue kallikrein and mRNA in various brain regions with a direct RIA nucleic acid hybridization using cDNA probe. In the RIA, rat urinary kallikrein-like activity was found pituitary pineal glands, hypothalamus, cerebral cortex, cerebellum, stem. Pituitary gland concentrations were significantly higher than those other regions. Only there significant difference concentration according to sex, glands from female rats showing levels 4-fold male rats. Kallikrein mRNAs all of about gland. Northern blot analyses show sex dimorphism mRNA, similar size submandibular kidney mRNA. castrated rats, whole content reduced 50% control value increased 1.7-fold testosterone replacement 18-fold 17 beta-estradiol treatment. Neither T4 nor cortisol affected rat, but decreased normal by 35%. When anterior or neurointermediate lobe extracts separately examined, immunoreactive 10.2- 1.3-fold respectively, lobes. Estradiol benzoate (30 micrograms/kg) administration 90- 22-fold, gonadectomized while it only 40-50% lobe. dot analysis, 5-fold cytoplasmic estradiol treatment 54-fold ovariectomized The data that indistinguishable thus far is widely distributed enzyme are comparable certain central sites. pituitaries differentially regulated estrogen.