Compositions and methods for nucleic acid sequencing

作者: Congcong Ma , Kevin Travers , Cheryl Heiner , Stephen Turner , Geoff Otto

DOI:

关键词: Double strandedComputational biologySequence determinationSequence (biology)Nucleic acid sequencingSense (molecular biology)GeneticsBiology

摘要: Compositions and methods for nucleic acid sequencing include template constructs that comprise double stranded portions in a partially or completely contiguous constructs, to provide redundant sequence determination through one both of sense antisense strands, iteratively the entire construct multiple times. Additional components are also optionally included within such constructs. Methods provided use preparation these as well compositions their application.

参考文章(161)
M. Hahn, W. Ansorge, H.K. Schackert, J. Plaschke, H. Voss, Doublex Sequencing in Molecular Diagnosis of Hereditary Diseases BioTechniques. ,vol. 24, pp. 838- 841 ,(1998) , 10.2144/98245DT06
William J. Efcavitch, Marie Sutherlin Causey, Methods for mutation detection ,(2007)
Ralph Rapley, The nucleic acid protocols : handbook Humana Press. ,(2000)
Wenonah Vercoutere, Stephen Winters-Hilt, Hugh Olsen, David Deamer, David Haussler, Mark Akeson, Rapid discrimination among individual DNA hairpin molecules at single-nucleotide resolution using an ion channel. Nature Biotechnology. ,vol. 19, pp. 248- 252 ,(2001) , 10.1038/85696
Leland J. Cseke, Donghern Kim, Peter B. Kaufman, William Wu, Handbook of Molecular and Cellular Methods in Biology and Medicine ,(1995)
Swati S. Ranade, Yangzhou Wang, Lei Xi, Bin Li, Method of making a paired tag library for nucleic acid sequencing ,(2009)