Isolation, Crystallization and Properties of a New Cellulase of Clostridium thermocellum Overproduced in Escherichia coli
作者： Gwennaël Joliff , Pierre Béguin , Michel Juy , Jacqueline Millet , Antoinette Ryter
DOI: 10.1038/NBT1086-896
关键词: Enzyme 、 Enterobacteriaceae 、 Biology 、 Biochemistry 、 Bacteria 、 Subcloning 、 Cellulase 、 Molecular cloning 、 Clostridium thermocellum 、 Escherichia coli 、 Biotechnology 、 Molecular medicine 、 Applied Microbiology and Biotechnology 、 Bioengineering 、 Biomedical engineering
摘要: Subcloning a Clostridium thermocellum DNA fragment carrying the celD gene resulted in high–level expression Escherichia coli of new cellulase termed endoglucanase D. With carboxymethylcellulose as substrate, D appears to be among most active cellulases hitherto described. The enzyme was purified from cytoplasmic granules, and crystals suitable for high–resolution X–ray analysis were obtained.
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