Attack-Phase Bdellovibrio bacteriovorus Responses to Extracellular Nutrients Are Analogous to Those Seen During Late Intraperiplasmic Growth

摘要: Bdellovibrio bacteriovorus is a predatory bacterium which lives by invading the periplasm of gram-negative bacteria and consuming them from within. This predator was thought to be dependent upon prey for nutrients since it lacks genes encoding critical enzymes involved in amino acid biosynthesis. study, however, found that planktonic attack-phase predators are not just nutrients, but rather, they respond surrounding medium and, subsequently, synthesize secrete proteases nutrient-dependent manner. The major secreted were identified through mass spectrometry analyses. Subsequent RT-qPCR analyses nutrient-induced similar those expressed within during late intraperiplasmic growth phase. Furthermore, RNA sequencing incubating cells nutritious environment short period time (4 h) changes its gene expression pattern status akin phase, with more than 94% previously as being intraperiplasmic-specific also induced nutrient broth this study. strong correlation between patterns hints availability hydrolyzed cell components likely stimulus controlling B. predation.