Herpes simplex virus vector-mediated delivery of neurturin rescues erectile dysfunction of cavernous nerve injury
作者： R Kato , D Wolfe , C H Coyle , J B Wechuck , P Tyagi
DOI: 10.1038/GT.2008.132
关键词: Blood pressure 、 Nerve injury 、 Biology 、 Surgery 、 Erectile dysfunction 、 Glial cell line-derived neurotrophic factor 、 Herpes simplex virus 、 Immunohistochemistry 、 Neurotrophic factors 、 Neurturin 、 Pathology
摘要: Neurturin (NTN), a member of glial cell line-derived neurotrophic factor (GDNF) family, is known as an important for penis-projecting neurons. We recently demonstrated significant protection from erectile dysfunction (ED) following replication-defective herpes simplex virus (HSV) vector-mediated GDNF delivery to the injured cavernous nerve. Herein, we applied HSV NTN this ED model. Rat nerve was bilaterally using clamp and dry ice. For HSV-treated groups, 20 microl vector stock administered directly damaged Delivery expressing both green fluorescent protein lacZ (HSV-LacZ) used control. Intracavernous pressure along with systemic arterial (ICP/AP) measured 2 4 weeks after injury. Fluorogold (FG) injected into penile crus 7 days before being killed assess neuronal survival. Four injury, rats treated HSV-NTN exhibited significantly higher ICP/AP compared untreated or control vector-treated groups. The group had more FG-positive major pelvic ganglion neurons than could be viable approach improvement
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