Gene expression in cadmium-tolerant Datura innoxia: detection and characterization of cDNAs induced in response to Cd2+.

摘要: The response of a metal tolerant plant to heavy stress involves number biochemical pathways. To investigate the overall molecular metal-tolerant heavy-metal exposure, suppressive subtractive hybridization was used create library enriched in cadmium-induced cDNAs from cadmium-tolerant Datura innoxia. Two differential screening steps were screen resulting 8 putative cadmium-specific out pool 94 clones. Reverse transcriptase polymerase chain reaction confirm that 4 these clones cadmium-specific, while other induced under heat shock or no treatment cells addition cadmium exposure. All sequenced and search for identification against GenBank. One had homology sulfur transferase-family protein Arabidopsis thaliana. possible link between this result plants is discussed.