Highly efficient conversion of 1-cyanocycloalkaneacetonitrile using a "super nitrilase mutant".
作者： Zhe Xu , Neng Xiong , Shu-Ping Zou , Yu-Xiao Liu , Zhi-Qiang liu
DOI: 10.1007/S00449-018-2049-2
关键词: Hydrolysis 、 Bioprocess 、 Industrial and production engineering 、 Hydrolase 、 Thermostability 、 Acidovorax facilis 、 Nitrilase 、 Chemistry 、 Substrate (chemistry) 、 Combinatorial chemistry
摘要: Nitrilase is the member of carbon–nitrogen hydrogen hydrolase superfamily, which has been widely used for hydrolysis nitriles into corresponding carboxylic acids. But most nitrilases are plagued by product inhibition in industrial application. In this study, a “super nitrilase mutant” with high activity, thermostability and improved tolerance from Acidovorax facilis ZJB09122 was characterized. Then, an efficient process developed employing whole cell recombinant E. coli conversion concentration 1-cyanocyclohexylacetonitrile-to-1-cyanocyclohexaneacetic acid, important intermediate gabapentin. Under optimized conditions, higher substrate concentrations such as 1.3 M, 1.5 M 1.8 M could be hydrolyzed 13.58 g DCW/L outstanding productivity (> 740 g/L/day). This study highly bioprocess preparation 1-cyanocyclohexaneacetic acid great potential
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