Over-expression of genes and proteins of ubiquitin specific peptidases (USPs) and proteasome subunits (PSs) in breast cancer tissue observed by the methods of RFDD-PCR and proteomics.
作者： Shishan Deng , Hongying Zhou , Ruohong Xiong , Youguang Lu , Dazhong Yan
DOI: 10.1007/S10549-006-9393-7
关键词: Molecular biology 、 Cancer 、 Gene expression 、 Proteasome 、 Ubiquitin-Protein Ligase E3A 、 PSMB5 、 Proteomics 、 USP9X 、 Ubiquitin 、 Biology
摘要: The ubiquitin-proteasome system facilitates the degradation of damaged proteins and regulators growth stress response. Alterations in this proteolytic are associated with a variety human pathologies. By restriction fragment differential display polymerase chain reaction (RFDD-PCR) matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF-TOF MS) based on two-dimensional polyacrylamide gel electrophoresis (2-DE), differentially expressed genes ubiquitin specific proteases (USPs), proteasome subuinits (PSs) protein ligase E3A (UBE3A) were analyzed between breast cancer adjacent normal tissues. Some them further verified as over-expression by immunohistochemical stain. Five subunits (PSs), including PSMB5, PSMD1, PSMD2, PSMD8 PSMD11, four USPs, USP9X, USP9Y, USP10 USP25, over-expressed (>3-fold) tissue compared to tissue, (>4-fold) PSMA1 SMT3A observed tissue. PSMD8, PSMD11 UBE3A action obviously enhanced cancer, selectively intervention may be useful method treating cancer.
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