Identification of known and novel genes in activated monocytes from patients with rheumatoid arthritis

摘要: Objective To define gene activation patterns of monocytes (MO) in patients with rheumatoid arthritis (RA). Methods A complementary DNA (cDNA) library was constructed from first-leukapheresis MO obtained an RA patient active disease; 32P-labeled cDNA (activated pool) and third-leukapheresis (nonactivated were used as probes for differential hybridization. Reverse transcriptase-polymerase chain reaction (RT-PCR) to assess additional 26 6 normal controls. Results Subtraction genes first- resulted 482 differentially expressed clones. In MO, these clones included the following: 1) interleukin-1alpha (IL-1alpha), IL-1beta, IL-6, tumor necrosis factor alpha, growth-related oncogene alpha (GROalpha)/melanoma growth-stimulatory activity, macrophage inflammatory protein 2/GRObeta, ferritin, alpha1-antitrypsin, lysozyme, transaldolase, Epstein-Barr virus-encoded RNA 1 (EBER-1)/EBER-2-associated-protein, thrombospondin 1, angiotensin receptor II (ATRII) C-terminal homolog, polymerase elongation (elongin); 2) two homologous functionally undefined (BSK-67 BSK-83); 3) three unknown sequences (BSK-66, 80, 89). differentiation (HOX-B3, thymosin-beta4, PU.1, glucocerebrosidase, MEL-18, glucose-6-phosphate dehydrogenase) 3 unknown/functionally sequences. Differential expression most activated pool confirmed leukapheresis samples 2 patients. patients, not only IL-1beta ATRII homolog significantly overexpressed (maximum 36-fold), but also 4 102-fold). Notably, messenger levels BSK-89 correlated positively erythrocyte sedimentation rate (ESR), whereas those BSK-83 negatively ESR C-reactive level. Conclusion The combined strategy subtraction semiquantitative RT-PCR may allow definition during different disease phases (including therapy-induced remission) identification novel pathogenetic relevance RA.