VdCrz1 is involved in microsclerotia formation and required for full virulence in Verticillium dahliae.
作者： Dianguang Xiong , Yonglin Wang , Chen Tang , Yulin Fang , Jingyi Zou
DOI: 10.1016/J.FGB.2015.07.011
关键词: Mutant 、 Virulence 、 Transcription factor 、 Biology 、 Gene expression 、 Verticillium dahliae 、 Verticillium wilt 、 Promoter 、 Microbiology 、 Zinc finger transcription factor
摘要: Calcium signaling plays crucial roles in ion stress tolerance, sporulation and pathogenicity fungi. Although the pathway mediated by calcineurin calcineurin-responsive zinc finger transcription factor Crz1 is well characterized other fungi, this not phytopathogenic fungus, Verticillium dahliae. To better understand role of calcineurin-dependent V. dahliae, an ortholog CRZ1, VdCrz1, was identified functionally. Transcriptional analysis VdCrz1 GFP expression driven promoter indicated that involved microsclerotia development. After targeted deletion formation melanin accumulation were impaired. Furthermore, ΔVdCrz1 mutants hypersensitive to high concentrations Ca(2+) cell wall-perturbing agents, such as sodium dodecyl sulfate. The addition Mg(2+) medium restores mutants. exhibited delayed wilt symptoms on smoke tree. These results suggest important signaling, wall integrity, development full virulence
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