Temperature modulates the Ca2+ current of HIT-T15 and mouse pancreatic β-cells

摘要: Abstract The effects of raising temperature on the Ca 2+ , currents insulin-secreting HIT and mouse pancreatic β-cells were studied. Currents measured in 3 mM containing solutions using standard whole-cell techniques. Increasing from 22°C to 35°C increased peak current amplitude, percent (fast) inactivation decreased time-to-peak current. responded same manner an imposed physiological burstwave with test-pulses: (i) application inactivated test-pulse at both high low temperatures; (ii) leveled off during plateau phase 20–22°C whereas there was apparent continual decay 33–35°C; (iii) recovery occurred interburst period temperatures. Application a without test-pulses before after addition 0.2 Cd resulted difference This activated hyperpolarized phase, activated, deactivated rapidly continually recovered interburst. Although strongly modified β-cell current, our work suggests that other channels, are likely be involved control islet bursts, particularly different In addition, effect cell may partly responsible for well-known dependence glucose-dependent secretion.