Creating and evaluating accurate CRISPR-Cas9 scalpels for genomic surgery
作者： Mehmet Fatih Bolukbasi , Ankit Gupta , Scot A Wolfe
DOI: 10.1038/NMETH.3684
关键词: Computational biology 、 Extramural 、 Biology 、 Genetics 、 CRISPR 、 Activity profile 、 Target site 、 Cas9 、 Genome editing 、 Genome
摘要: The simplicity of site-specific genome targeting by type II clustered, regularly interspaced, short palindromic repeat (CRISPR)-Cas9 nucleases, along with their robust activity profile, has changed the landscape editing. These favorable properties have made CRISPR-Cas9 system technology choice for sequence-specific modifications in vertebrate systems. For many applications, whether focus is on basic science investigations or therapeutic efficacy, and precision are important considerations when one choosing a nuclease platform, target site delivery method. Here we review recent methods increasing accuracy Cas9 assessing extent off-target cleavage events.
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Pankaj K. Mandal, Leonardo M.R. Ferreira, Ryan Collins, Torsten B. Meissner, Christian L. Boutwell, Max Friesen, Vladimir Vrbanac, Brian S. Garrison, Alexei Stortchevoi, David Bryder, Kiran Musunuru, Harrison Brand, Andrew M. Tager, Todd M. Allen, Michael E. Talkowski, Derrick J. Rossi, Chad A. Cowan, Efficient ablation of genes in human hematopoietic stem and effector cells using CRISPR/Cas9 Cell Stem Cell. ,vol. 15, pp. 643- 652 ,(2014) , 10.1016/J.STEM.2014.10.004
Baohui Chen, Luke A. Gilbert, Beth A. Cimini, Joerg Schnitzbauer, Wei Zhang, Gene-Wei Li, Jason Park, Elizabeth H. Blackburn, Jonathan S. Weissman, Lei S. Qi, Bo Huang, Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System Cell. ,vol. 155, pp. 1479- 1491 ,(2013) , 10.1016/J.CELL.2013.12.001
F. Ann Ran, Le Cong, Winston X. Yan, David A. Scott, Jonathan S. Gootenberg, Andrea J. Kriz, Bernd Zetsche, Ophir Shalem, Xuebing Wu, Kira S. Makarova, Eugene V. Koonin, Phillip A. Sharp, Feng Zhang, In vivo genome editing using Staphylococcus aureus Cas9 Nature. ,vol. 520, pp. 186- 191 ,(2015) , 10.1038/NATURE14299
Yan Zhang, Nadja Heidrich, Biju Joseph Ampattu, Carl W. Gunderson, H. Steven Seifert, Christoph Schoen, Jörg Vogel, Erik J. Sontheimer, Processing-Independent CRISPR RNAs Limit Natural Transformation in Neisseria meningitidis Molecular Cell. ,vol. 50, pp. 488- 503 ,(2013) , 10.1016/J.MOLCEL.2013.05.001
Van Trung Chu, Timm Weber, Benedikt Wefers, Wolfgang Wurst, Sandrine Sander, Klaus Rajewsky, Ralf Kühn, Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nature Biotechnology. ,vol. 33, pp. 543- 548 ,(2015) , 10.1038/NBT.3198
John A Zuris, David B Thompson, Yilai Shu, John P Guilinger, Jeffrey L Bessen, Johnny H Hu, Morgan L Maeder, J Keith Joung, Zheng-Yi Chen, David R Liu, Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo Nature Biotechnology. ,vol. 33, pp. 73- 80 ,(2015) , 10.1038/NBT.3081
Xuebing Wu, David A Scott, Andrea J Kriz, Anthony C Chiu, Patrick D Hsu, Daniel B Dadon, Albert W Cheng, Alexandro E Trevino, Silvana Konermann, Sidi Chen, Rudolf Jaenisch, Feng Zhang, Phillip A Sharp, Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells Nature Biotechnology. ,vol. 32, pp. 670- 676 ,(2014) , 10.1038/NBT.2889
Yanfang Fu, Jennifer A Foden, Cyd Khayter, Morgan L Maeder, Deepak Reyon, J Keith Joung, Jeffry D Sander, High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells Nature Biotechnology. ,vol. 31, pp. 822- 826 ,(2013) , 10.1038/NBT.2623
Hind Ghezraoui, Marion Piganeau, Benjamin Renouf, Jean-Baptiste Renaud, Annahita Sallmyr, Brian Ruis, Sehyun Oh, Alan E. Tomkinson, Eric A. Hendrickson, Carine Giovannangeli, Maria Jasin, Erika Brunet, Chromosomal Translocations in Human Cells Are Generated by Canonical Nonhomologous End-Joining Molecular Cell. ,vol. 55, pp. 829- 842 ,(2014) , 10.1016/J.MOLCEL.2014.08.002
Matthew C. Canver, Daniel E. Bauer, Abhishek Dass, Yvette Y. Yien, Jacky Chung, Takeshi Masuda, Takahiro Maeda, Barry H. Paw, Stuart H. Orkin, Characterization of Genomic Deletion Efficiency Mediated by Clustered Regularly Interspaced Palindromic Repeats (CRISPR)/Cas9 Nuclease System in Mammalian Cells Journal of Biological Chemistry. ,vol. 289, pp. 21312- 21324 ,(2014) , 10.1074/JBC.M114.564625