Identification of cell surface receptors for murine macrophage inflammatory protein-1 alpha.
作者： B. S. Kwon , H. E. Broxmeyer , K.-O. Oh , Young-June Kim , Kack-Kyun Kim
DOI:
关键词: Molecular biology 、 Cell culture 、 T cell 、 Interleukin 10 receptor, alpha subunit 、 Endocrinology 、 Interleukin 5 receptor alpha subunit 、 Biology 、 Internal medicine 、 Liver X receptor alpha 、 Receptor 、 Cell surface receptor 、 Alpha (ethology)
摘要: We have produced recombinant proteins for a cytokine, L2G25BP (macrophage inflammatory protein-1 alpha) (MIP-1 alpha). By using the protein (rMIP-1 alpha), receptors MIP-1 alpha were identified on Con A-stimulated and unstimulated CTLL-R8, T cell line, LPS-stimulated RAW 264.7, macrophage line. The 125I-rMIP-1 binds to receptor in specific saturable manner. Scatchard analysis indicated single class of high affinity receptor, with Kd approximately 1.5 x 10(-9) M 1200 binding sites/Con CTLL-R8 0.9 380 sites/RAW 264.7 cell. was inhibited by unlabeled rMIP-1 dose-dependent manner, but not IL-1 or IL-2. proliferation cells. Rabbit anti-rMIP-1 antibodies blocked growth-inhibitory effect
jimmunol.org 参考文章(0)