Seminal Cell-Free DNA Assessment as a Novel Prostate Cancer Biomarker.
作者： Giovanni Ponti , Monia Maccaferri , Mauro Mandrioli , Marco Manfredini , Salvatore Micali
DOI: 10.1007/S12253-018-0416-6
关键词: Cell-free fetal DNA 、 Liquid biopsy 、 Cancer cell 、 Semen 、 Prostate cancer screening 、 Fragmentation (cell biology) 、 Cancer research 、 Prostate cancer 、 Medicine 、 Biomarker (medicine)
摘要: Cell-free DNA (cfDNA) includes circulating fragments, which can be obtained from different human biological samples. cfDNA originates either apoptotic and/or necrotic cells or is actively secreted by cancer cells. As yet, a quantification and size distribution assessment of seminal plasma prostate patients has never been assessed. To discover novel, sensitive, non-invasive biomarker cancer, through the fluorometric electrophoretic analysis in compared to healthy individuals. The concentration was 2243.67 ± 1758 ng/μl, 57.7 ± 4.8 ng/μl individuals (p < 0.05). Electrophoresis sites patterns were different; ladder fragmentation associated with Human fluid valuable source setting liquid biopsy procedures for identification novel oncological biomarkers. Seminal significantly more concentrated than that age-matched, controls. Fluorometric measurement allow reliable characterization cfDNA, used routinely screening programs.
springer.com
core.ac.uk 
sci-hub.se 参考文章(9)
Michael J. Duffy, PSA in screening for prostate cancer: more good than harm or more harm than good? Advances in Clinical Chemistry. ,vol. 66, pp. 1- 23 ,(2014) , 10.1016/B978-0-12-801401-1.00001-3
M Stroun, J Lyautey, C Lederrey, A Olson-Sand, P Anker, About the possible origin and mechanism of circulating DNA apoptosis and active DNA release. Clinica Chimica Acta. ,vol. 313, pp. 139- 142 ,(2001) , 10.1016/S0009-8981(01)00665-9
Frank O. Fackelmayer, Rolf Knippers, Sabine Englisch, Dieter Hardt, Sabine Jahr, Hannes Hentze, Rolf-Dieter Hesch, DNA Fragments in the Blood Plasma of Cancer Patients: Quantitations and Evidence for Their Origin from Apoptotic and Necrotic Cells Cancer Research. ,vol. 61, pp. 1659- 1665 ,(2001)
Hong-Gang Li, Shi-Yun Huang, Hui Zhou, Ai-Hua Liao, Cheng-Liang Xiong, Quick recovery and characterization of cell-free DNA in seminal plasma of normozoospermia and azoospermia: implications for non-invasive genetic utilities. Asian Journal of Andrology. ,vol. 11, pp. 703- 709 ,(2009) , 10.1038/AJA.2009.65
Jun-Liang Lu, Zhi-Yong Liang, Circulating free DNA in the era of precision oncology: Pre- and post-analytical concerns Chronic Diseases and Translational Medicine. ,vol. 2, pp. 223- 230 ,(2016) , 10.1016/J.CDTM.2016.12.001
F. Costa, F. Barbisan, C.E. Assmann, N.K.F. Araújo, A.R. de Oliveira, J.P. Signori, F. Rogalski, B. Bonadiman, M. S. Fernandes, I.B.M. da Cruz, Seminal cell-free DNA levels measured by PicoGreen fluorochrome are associated with sperm fertility criteria. Zygote. ,vol. 25, pp. 111- 119 ,(2017) , 10.1017/S0967199416000307
Maxim V. Zagoskin, Richard E. Davis, Dmitry V. Mukha, Double Stranded RNA in Human Seminal Plasma. Frontiers in Genetics. ,vol. 8, pp. 154- 154 ,(2017) , 10.3389/FGENE.2017.00154
Giovanni Ponti, Monia Maccaferri, Marco Manfredini, Shaniko Kaleci, Mauro Mandrioli, Giovanni Pellacani, Tomris Ozben, Roberta Depenni, Giampaolo Bianchi, Giacomo Maria Pirola, Aldo Tomasi, The value of fluorimetry (Qubit) and spectrophotometry (NanoDrop) in the quantification of cell-free DNA (cfDNA) in malignant melanoma and prostate cancer patients Clinica Chimica Acta. ,vol. 479, pp. 14- 19 ,(2018) , 10.1016/J.CCA.2018.01.007
G. Ponti, M. Maccaferri, M. Manfredini, M. Cotugno, G. Pellacani, A. Conti, S. Micali, M. Mandrioli, A. Tomasi, Seminal cell-free DNA molecular profile as a novel diagnostic and prognostic prostate cancer biomarkers Medical Hypotheses. ,vol. 114, pp. 69- 69 ,(2018) , 10.1016/J.MEHY.2018.02.034