T-cell receptor beta-chain expression: dependence on relatively few variable region genes
作者： M. Behlke , D. Spinella , H. Chou , W Sha , D. Hartl
关键词: Beta (finance) 、 T-cell receptor 、 Complementary DNA 、 Southern blot 、 Genetics 、 Gene 、 Somatic hypermutation 、 Molecular biology 、 Gene pool 、 T-Cell Receptor Beta Chain 、 Biology
摘要: Fifteen independently isolated complementary DNA clones that contain T-cell receptor (TCR) V beta genes were sequenced and found to represent 11 different genes. When compared with known sequences, 14 could be defined from a total of 25 DNA's; therefore involved repeated usage previously identified beta's. Based on these data, we calculate maximum likelihood estimate the number expressed germline 18 an upper 95 percent confidence bound 30 Southern blot analysis has shown most belong single element subfamilies which show very limited interstrain polymorphism. The TCR beta-chain diversity appears generated gene pool primarily by extensive variability at variable-diversity-joining (V-D-J) junctional site, no evidence for involvement somatic hypermutation.
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