Horizontal genetic exchange of chromosomally encoded markers between Campylobacter jejuni cells.

摘要: Many epidemiological studies provide us with the evidence of horizontal gene transfer (HGT) contributing to bacterial genomic diversity that benefits populations increased ability adapt dynamic environments. Campylobacter jejuni, a major cause acute enteritis in U.S., often linked severe post-infection neuropathies, has been reported exhibit non-clonal population structure and comparatively higher strain-level genetic variation. In this study, we HGT chromosomally encoded markers between C. jejuni cells biphasic MH medium. We used two NCTC-11168 mutants harbouring distinct antibiotic-resistance genes [chloramphenicol (Cm) kanamycin (Km)] present at different neutral loci. Cultures both marker strains were mixed together incubated for 5 hrs, then plated on agar plates supplemented antibiotics. The recombinant double antibiotic generated frequency 0.02811 ± 0.0035% parental strains. PCR assays using locus-specific primers confirmed was through homologous recombination. Also, addition chicken cecal content recombination efficiency approximately up 10-fold as compared medium (control) P < 0.05. Furthermore, treating co-culture DNase I decreased available DNA, which turn significantly reduced by 99.92% (P 0.05). cell-free supernatant 16 hrs-culture Wild-type template found DNA sequences from six regions easily amplified, indicating presence released chromosomal culture supernatant. Our findings suggest is facilitated gut environment vivo diversity. Additionally, might have an active mechanism release its into extracellular environment, further expediting populations.