Vibrio cholerae O1 strains are facultative intracellular bacteria, able to survive and multiply symbiotically inside the aquatic free-living amoeba Acanthamoeba castellanii

摘要: Vibrio cholerae species are extracellular, waterborne, gram-negative bacteria that overwhelmed by predators in aquatic environments. The unencapsulated serogroup V. O1 and encapsulated O139 cause epidemic pandemic outbreaks of cholera. It has recently been shown the free-living amoeba Acanthamoeba castellanii is not a predator to O139; rather, an intracellular compatibility with this host. aim study was examine ability classical El Tor strains grow survive A. castellanii. interaction between studied means cell counts viable absence or presence amoebae. count intracellularly growing estimated utilizing gentamicin assay. Confocal microscopy electron were used determine localization results showed grew survived cytoplasm trophozoites, also found cysts facultative behaviour possible role as environmental host species.