Robust immunohistochemical staining of several classes of proteins in tissues subjected to autolysis.

摘要: Despite the common use of immunohistochemistry in autopsy tissues, stability most proteins over extended time periods is unknown. The robustness signal for 16 (MMP1, MMP2, MMP3, MMP9, TIMP1, TIMP2, TIMP3, AGER, MSR, SCARB1, OLR1, CD36, LTF, LGALS3, LYZ, and DDOST) two measures advanced glycation end products (AGE, CML) was evaluated. Two formalin-fixed, paraffin-embedded human tissue arrays containing tissues each were created to evaluate 48 hr autolysis a warm or cold environment. For these classes proteins, matrix metalloproteinases their inhibitors, scavenger receptors, product we saw no systematic diminution intensity during period 24 hr. Analysis performed by independent observers confirmed subset digital analysis Western blotting. We conclude that degrade slowly faithfully maintain characteristics at least 24-hr interval devitalized tissues. This study supports with short postmortem intervals immunohistochemical studies diseases such as diabetic vascular disease, cancer, Alzheimer's atherosclerosis, other pathological states. manuscript contains online supplemental material http://www.jhc.org. Please visit this article view materials.