Simultaneous determination of catecholamines and unconjugated 3,4-dihydroxyphenylacetic acid in brain tissue by ion-pairing reverse-phase high-performance liquid chromatography with electrochemical detection
作者： Ivan N. Mefford , Mark Gilberg , Jack D. Barchas
DOI: 10.1016/0003-2697(80)90101-3
关键词: Desorption 、 Catecholamine 、 Adsorption 、 3,4-Dihydroxyphenylacetic acid 、 Chemistry 、 Monoamine oxidase 、 In vivo 、 Dopamine 、 Chromatography 、 High-performance liquid chromatography
摘要: A rapid, sensitive method was developed for the simultaneous assay of catecholamines and 3,4-dihydroxyphenylacetic acid in rat brain tissue. The is simple, involving only tissue disruption, adsorption catechols onto alumina, desorption, injection into a reverse-phase high-performance liquid chromatography system. Selectivity high sensitivity are obtained using electrochemical detection. addition determination to assays allows one observe effects pharmacological maniqulations on vivo monoamine oxidase activity and/or turnover dopamine as well catecholamine concentrations.
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