Characterization of polycyclic aromatic compounds in diesel exhaust particulate extract responsible for aryl hydrocarbon receptor activity

摘要: Chemical fractions of a model diesel exhaust particulate extract, notably the fraction containing polycyclic aromatic hydrocarbons (PAH) (Fraction II), mono-nitro PAH III), and dinitro-PAH IV) have been shown to displace binding 2,3,7,8-tetrachloro[1,6-[3H]]dibenzo-p-dioxin (TCDD) from rat liver cytosol aryl hydrocarbon (Ah) receptor. The Ah receptor activity twenty-seven PAH, identified in Fraction II recombined same proportions as those observed original fraction, was not significantly different that II, suggesting this, can be explained by components. In addition, these two samples had ability induce expression an target gene, CYP450lA1, indicated solution hybridisation assay mRNA levels mouse Hepa 1c1c7 cells. On screening individual reference following compounds displayed IC50 values for < l μM: benzo(a)-fluorene, benz(a)anthracene, chrysene, benzo(b)fluoranthene, benzo(a)pyrene indeno(1,2,3-cd)pyrene. These six also induced CYP4501A1 range 2–6-fold above baseline levels. Further studies demonstrated could presence PAR chromatographic behaviour affinity indicate 3-nitroperylene, 6-nitrobenzo(a)pyrene 7-nitrobenz(a)-anthracene are candidate responsible high III.