Polynucleotides encoding metal ion affinity peptides and related products
作者： Grigoriy S. Tchaga , George G. Jokhadze
DOI:
关键词: Recombinant DNA 、 Metal 、 Side chain 、 Stereochemistry 、 Amino acid 、 Peptide 、 Amide 、 Fusion protein 、 Chemistry 、 Polynucleotide
摘要: The present invention provides metal ion affinity peptides, fusion proteins comprising and polynucleotides encoding the proteins. A feature of subject is that peptide has a formula selected from group consisting of: 1: (His-X 1 -X 2 ) n1 -(His-X 3 4 5 n2 6 n3 , wherein each X independently an amino acid with aliphatic or amide side chain, basic chain (except His) acidic are 1–3, 1–5; 2: (His-Asn) n where n=3 to 10; 3: having 10. further recombinant vectors polynucleotides, host cells vectors. methods kits for purifying protein peptide.
lens.org 参考文章(23)
Jennifer Hopp, Paul Nelson, Grigoriy Chaga, Immobilized metal ion affinity chromatography on Co2+-carboxymethylaspartate–agarose Superflow, as demonstrated by one-step purification of lactate dehydrogenase from chicken breast muscle Biotechnology and Applied Biochemistry. ,vol. 29, pp. 19- 24 ,(1999) , 10.1111/J.1470-8744.1999.TB01144.X
Willem P. C. Stemmer, Michele C. Smith, Gary S. David, Leslie D. Anderson, James A. Cook, Mary S. Kasher, Susan M. Hochschwender, Immobilization of biologically active molecules by changing the Oxidation state of a chelated transition metal ion ,(1992)
Michele C. Smith, Charles Pidgeon, Process for purifying proteins and compounds useful in such process ,(1984)
Satish K. Sharma, Process of purifying recombinant proteins and compounds useful in such process ,(1994)
M Moser, G Menz, K Blaser, R Crameri, Recombinant expression and antigenic properties of a 32-kilodalton extracellular alkaline protease, representing a possible virulence factor from Aspergillus fumigatus. Infection and Immunity. ,vol. 62, pp. 936- 942 ,(1994) , 10.1128/IAI.62.3.936-942.1994
Clark F. Ford, Ilari Suominen, Charles E. Glatz, Fusion tails for the recovery and purification of recombinant proteins Protein Expression and Purification. ,vol. 2, pp. 95- 107 ,(1991) , 10.1016/1046-5928(91)90057-P
K Itakura, T Hirose, R Crea, A. Riggs, H. Heyneker, F Bolivar, H. Boyer, Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin Science. ,vol. 198, pp. 1056- 1063 ,(1977) , 10.1126/SCIENCE.412251
M.C. Smith, T.C. Furman, J.A. Cook, T. Ingolia, H. Hsiung, Chelating peptide-immobilized metal ion affinity chromatography Journal of Inorganic Biochemistry. ,vol. 36, pp. 277- ,(1989) , 10.1016/0162-0134(89)84385-5
J. Germino, J. G. Gray, H. Charbonneau, T. Vanaman, D. Bastia, Use of gene fusions and protein-protein interaction in the isolation of a biologically active regulatory protein: the replication initiator protein of plasmid R6K. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 80, pp. 6848- 6852 ,(1983) , 10.1073/PNAS.80.22.6848
Grigoriy Cℏaga, Dmitry E Bochkariov, George G Jokhadze, Jennifer Hopp, Paul Nelson, Natural poly-histidine affinity tag for purification of recombinant proteins on cobalt(II)-carboxymethylaspartate crosslinked agarose Journal of Chromatography A. ,vol. 864, pp. 247- 256 ,(1999) , 10.1016/S0021-9673(99)01008-0