Bioethanol production from lignocellulosic feedstock using aqueous ammonia pretreatment and simultaneous saccharification and fermentation (SSF): process development and optimization

摘要: An integrated bioconversion process was developed to convert corn-stover derived pentose and hexose ethanol effectively. In this study, corn stover pretreated by soaking in aqueous ammonia (SAA), which retained glucan (~100%) xylan (>80%) the solids. The carbohydrates-rich converted via two-phase simultaneous saccharification fermentation (TPSSF). This single-reactor employed sequential (SSF), i.e., conversion using recombinant Escherichia coli KO11 first phase, followed with Saccharomyces cerevisiae D5A second phase. 88% of digestibility achieved through synergistic action xylanase endo-glucanase minimal hydrolysis (10.5%). Overall, TPSSF 12-h SAA-treated resulted highest concentration (22.3 g/L), equivalent 84% theoretical yield based on total carbohydrates (glucan+xylan) untreated stover.