Transcriptome-based screening of ion channels and transporters in a migratory chondroprogenitor cell line isolated from late-stage osteoarthritic cartilage.

摘要: Chondrogenic progenitor cells (CPCs) may be used as an alternative source of with potentially superior chondrogenic potential compared to mesenchymal stem (MSCs), and could exploited for future regenerative therapies targeting articular cartilage in degenerative diseases such osteoarthritis (OA). In this study, we hypothesised that CPCs derived from OA characterised by a distinct channelome. First, global transcriptomic analysis using Affymetrix microarrays was performed. We studied the profiles those ion channels transporter families relevant chondroprogenitor cell physiology. Following validation microarray data quantitative reverse transcription-polymerase chain reaction, examined role calcium-dependent potassium observed functional large-conductance calcium-activated (BK) involved maintenance phenotype. line our very recent results, found KCNMA1 gene upregulated currents attributed BK channel. The channel inhibitor paxilline significantly inhibited proliferation, increased expression osteogenic transcription factor RUNX2, enhanced migration parameters, completely abolished spontaneous Ca2+ events CPCs. Through characterisation their channelome demonstrate are population but highly similar MSCs many respects. This study adds key mechanistic in-depth phenotype context regeneration.