Recombinant bovine spleen myristoyl CoA: protein N-myristoyltransferase.
作者： Rajala VS Raju , Raju SS Datla , Rakesh Kakkar , Rajendra K Sharma , None
关键词: Fast protein liquid chromatography 、 Protein kinase A 、 Biochemistry 、 Histidine 、 Biology 、 Peptide sequence 、 Peptide 、 Fusion protein 、 Enzyme 、 Enzyme assay 、 Molecular biology
摘要: Myristoyl-CoA:protein N-myristoyltransferase (NMT) is an essential eukaryotic enzyme that catalyzes the co-translational transfer of myristate to NH2-terminal glycine residue a number important proteins diverse function. Recently, we have isolated full length cDNA encoding bovine spleen NMT [27] was cloned and expressed in E. coli, resulting expression functionally active 50 kDa NMT. Using combination SP-Sepharose fast flow Mono S protein liquid chromatography, purified 20-fold with high yield. The (sNMT) fusion exhibited apparent molecular weight 53 on SDS-PAGE. Upon cleavage by Enterokinase sNMT without loss catalytic activity. two synthetic peptide substrates based N-terminal sequence pp60src (GSSKSKMR) cAMP dependent kinase (GNAAAKKRR) different kinetic parameters Km values 40 200 μM. Recombinant also potently inhibited Ni2+ (histidine binder) concentration manner half maximal inhibition 280 & mgr;M. coli homogenous showed
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